Scientist, Applications Sample Preparation
Glyphic Biotechnologies
<div class="content-intro"><h3><strong>About Glyphic:</strong></h3> <p>At Glyphic Biotechnologies, we plan to create the protein revolution for which scientists and researchers have been waiting. We are developing a massively parallel, single-molecule proteome sequencing platform that will transform life science discovery and usher in a new era of insights into human biology and disease. To date, we have raised &gt;$80M from venture partners and non-dilutive grant funding to achieve our vision of next generation proteome sequencing.</p></div><div class="ql-block ql-align-center" data-block-id="block-fc3814ff-f527-4170-923b-5eb429bbd977"> <p><strong>What we are looking for in you</strong></p> <p>We are seeking a technically driven, detail-oriented, and collaborative Scientist to apply and optimize sample preparation workflows across Glyphic’s single-molecule protein sequencing platform (ProSE), as well as complementary DNA sequencing, RNA sequencing, and mass spectrometry-based proteomics workflows. Reporting to the Director of Assay Integration, you will execute and refine established sample preparation protocols across a broad range of biological inputs and generate high-quality data that supports ProSE performance benchmarking and Glyphic’s broader multi-omic strategy, combining ProSE, RNA-seq, DNA-seq, Ribo-seq, and mass spectrometry to identify neoantigens, biomarkers, and drug targets</p> <p>The ideal candidate is comfortable executing and troubleshooting multi-step protocols across both nucleic acid- and protein-based workflows, rapidly adapting established methods to new sample types, and communicating results clearly across multidisciplinary teams. A track record of hands-on sample prep execution across proteomics and NGS platforms, and comfort operating in high-throughput environments, will be key to making an immediate impact.</p> <p>This is a full-time, exempt, in-person position with work conducted at our Berkeley, CA location.</p> <p><strong>What you’ll do</strong></p> <p><span style="text-decoration: underline;">Sample Preparation &amp; Assay Execution</span></p> <ul> <li>Evaluate and process incoming biological sample inputs,&nbsp; including cultured cells, fixed cells, tissue sections, blood, and Key Opinion Leader (KOL) samples — assessing sample-specific constraints and analytical requirements before entering the workflow.</li> <li>Extract and quantify nucleic acids and proteins from these samples, applying established extraction methods across diverse matrices.</li> <li>Apply and adapt enrichment, depletion, and dynamic-range management protocols to improve input quality for low-abundance targets and optimize ProSE and NGS sequencing yield.</li> <li>Perform core molecular biology reactions — buffer preparation, reagent formulation, enzymatic reactions, gel electrophoresis, PCR/qPCR, hybridization-based assays, and amplification workflows — to prepare samples for downstream analysis.</li> <li>Generate sequencing- and analysis-ready outputs: NGS libraries for DNA-seq, RNA-seq, and ribosome profiling across a broad set of platforms, and processed samples for ProSE and mass spectrometry-based proteomic readouts.</li> <li>Support the generation and sourcing of standard reference materials using cell culture techniques.</li> </ul> <p><span style="text-decoration: underline;">Assay Optimization &amp; Data Analysis</span></p> <ul> <li>Test protocol variables — reagent concentrations, incubation times, temperature conditions, wash conditions, sample preparation steps, and workflow timing — to optimize assay performance.</li> <li>Generate and analyze high-quality e