Staff / Senior Scientist, Assay Development (Chemistry)
Glyphic Biotechnologies
<div class="content-intro"><h3><strong>About Glyphic:</strong></h3> <p>At Glyphic Biotechnologies, we plan to create the protein revolution for which scientists and researchers have been waiting. We are developing a massively parallel, single-molecule proteome sequencing platform that will transform life science discovery and usher in a new era of insights into human biology and disease. To date, we have raised &gt;$80M from venture partners and non-dilutive grant funding to achieve our vision of next generation proteome sequencing.</p></div><div class="ql-block ql-align-center" data-block-id="block-fc3814ff-f527-4170-923b-5eb429bbd977"> <p><strong>What we are looking for in you</strong></p> <p>We are seeking a Senior or Staff Scientist to join our Assay Development team at a critical stage in platform development. Reporting to the Director of Assay Development, you will be a hands-on expert working at the chemistry-assay interface — developing the chemical methods that enable reliable, high-efficiency sample loading into our expansion assay, and building the novel conjugation, cleavage, and stabilization workflows that power our single-molecule proteome sequencing platform. You will work closely with the Chemistry team to develop sample input methods for new protein and peptide types, including side-chain protection strategies, encoder loading routes, and peptide-from-protein handoff approaches.</p> <p>The ideal candidate brings deep expertise in peptide chemistry, bioorthogonal and click conjugation strategies, and cleavage chemistry development — with a track record of building new chemical methods for complex, multi-component systems. You combine a synthetic chemist's mechanistic rigor with sensitivity to how chemistry performs inside a demanding multi-cycle assay environment.</p> <p>This is a full-time, exempt, in-person position with work conducted at our Berkeley, CA location.</p> <p><strong>What you’ll do</strong></p> <p><span style="text-decoration: underline;">Expansion assay chemistry &amp; sample loading</span></p> <ul> <li>Optimize bioconjugation methods for peptide loading into the expansion assay; establish robust, high-efficiency loading protocols that perform reliably across diverse peptide populations and physicochemical characteristics.</li> <li>Identify, characterize, and optimize peptide labeling during the ProSE sequencing assay, leveraging mechanistic understanding of Edman chemistry and amino acid side-chain reactivity and stability.</li> <li>Develop reagents and methods to maximize the stability of peptides, DNA, and peptide-DNA chimeras throughout the ProSE sequencing assay.</li> <li>Partner with the Chemistry team to develop amino acid side-chain protection chemistries.</li> <li>Evaluate compatibility of new chemistries with existing assay components, reagents, and multi-cycle sequencing conditions.</li> <li>Evaluate emerging chemistries from the literature and internal pipeline; synthesize and test candidate reagents.</li> </ul> <p><span style="text-decoration: underline;">Novel assay chemistry workflows</span>&nbsp;</p> <ul> <li>Design and determine the order of operations for chemical manipulations within the assay workflow — including decisions on when specific steps occur on-bead versus off-bead, and how side-chain protection and deprotection strategies are sequenced relative to assay cycles — to ensure compatibility, efficiency, and analytical interpretability.</li> <li>Optimize selective cleavage chemistry workflows for use within the sequencing assay.</li> <li>Optimize for efficiency, selectivity, and compatibility with assay c