Staff / Senior Scientist, Bioconjugation Chemistry
Glyphic Biotechnologies
<div class="content-intro"><h3><strong>About Glyphic:</strong></h3> <p>At Glyphic Biotechnologies, we plan to create the protein revolution for which scientists and researchers have been waiting. We are developing a massively parallel, single-molecule proteome sequencing platform that will transform life science discovery and usher in a new era of insights into human biology and disease. To date, we have raised &gt;$80M from venture partners and non-dilutive grant funding to achieve our vision of next generation proteome sequencing.</p></div><div class="ql-block ql-align-center" data-block-id="block-fc3814ff-f527-4170-923b-5eb429bbd977"> <p><strong>What we are looking for in you</strong></p> <p>We are seeking a Senior or Staff Scientist, Bioconjugation to join our Chemistry team at a pivotal stage in assay development. Reporting to the Senior Director of Chemistry, you will be a hands-on expert who designs, develops, and executes bioconjugation strategies for peptide and oligonucleotide components that are central to our single-molecule proteome sequencing assay.</p> <p>The ideal candidate brings deep expertise in protein and peptide bioconjugation, site-specific chemical modification of amino acid sidechains, and oligonucleotide-biomolecule conjugation, with a track record of working in complex, multi-component systems (e.g., ADCs, therapeutic bioconjugates, or new bioconjugated components for assays). A successful candidate will have an innovative method-development mindset and will effectively build new approaches to making bioconjugates. This individual will thrive in a cross-functional environment where chemical solutions must be tightly integrated into assay design.</p> <p>This role offers the unique opportunity to develop first-in-class conjugation chemistry that enables single-molecule protein sequencing.&nbsp;</p> <p>This is a full-time, exempt, in-person position with work conducted at our Berkeley, CA location.</p> <p><strong>What you’ll do</strong></p> <p><strong>Bioconjugation strategies:</strong></p> <ul> <li>Partner with sample prep and assay development to screen and select conjugation chemistries for peptides and oligonucleotides that enable single molecule protein sequencing</li> <li>Design bioconjugation workflows using NHS-ester chemistry, maleimides, isothiocyanate chemistry, and click chemistry (CuAAC, SPAAC, IEDDA)&nbsp;</li> <li>Prepare bioconjugation reagents to enable&nbsp; studies from proof-of-concept through prototype-kitting.</li> </ul> <p><strong>Peptide chemistry:</strong></p> <ul> <li>Develop labeling strategies based on Edman Chemistry</li> <li>Develop and apply selective C-terminal and N-terminal conjugation chemistries</li> <li>Develop site-specific labeling strategies for amino acid sidechains (Cys, Lys, Arg, Tyr, Trp, His, Asp/Glu) on native peptides. Screen protecting groups to enhance amino acid signal in the protein sequencing assay</li> <li>Enable on-bead workflow to label and release peptides for interrogation in the sequencing assay</li> </ul> <p><strong>Synthesize, characterize, and scale:</strong></p> <ul> <li>Synthesize and purify peptide and oligonucleotide conjugates for assay testing</li> <li>Characterize conjugation efficiency, purity, and stability using tools such as HPLC, LC-MS/MS, NMR, CE, UV/Vis, and FTIR</li> <li>Scale conjugate synthesis from R&amp;D quantities to prototype-grade supply for internal testing, with attention to reproducibility, shelf-life, and fit-for-purpose quality</li> </ul> <p><strong>Collaborate and document:</stron